Chang lab @ Penn

VIDEOS


(Narrated, please turn on speakers)

New mechanistic hypotheses for the extension and retraction of the bacterial type IVa pilus from working models constructed by electron cryotomography, subtomogram averaging, and mutant analysis.

From the publication: Chang, Y.-W., et al. Science (2016). “Architecture of the type IVa pilus machine


The process of using electron cryotomography, subtomogram averaging, mutant analysis, and previous knowledge of connectivities to build a working model of the Myxococcus xanthus type IV pilus motility machine, providing insights into assembly, structure, and function.

From the publication: Chang, Y.-W., et al. Science (2016). “Architecture of the type IVa pilus machine

Same video as the left one, but slower and with narration. Please turn on speakers.

From the publication: Chang, Y.-W., et al. Science (2016). “Architecture of the type IVa pilus machine


Process of the correlated cryo-PALM and ECT to capture type VI secretion system structures in vivo.

From the publication: Chang, Y. -W., et al. Nature Methods (2014). “Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography


Electron cryotomography, 3D reconstruction, and segmentation of an intact bacterial cell highlighting cellular features and showing how, in a growing number of cases, atomic models can now be fit into their context within the cell

From the Publication: Oikonomou, C.M., Chang, Y.-W., and Jensen, G.J. Nature Review Microbiology (2016). “A new view into prokaryotic cell biology from electron cryotomography

Process of the correlated cryo-PALM and ECT to capture type VI secretion system structures in vivo.

From the publication: Chang, Y. -W., et al. Nature Methods (2014). “Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography