Chang lab @ Penn


Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Debnath Ghosal, Kwangcheol Jeong, Yi-Wei Chang, Jacob Gyore, Lin Teng, Adam Gardner, Joseph Vogel, Grant Jensen

Nature Microbiology

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

Lam Nguyen, Catherine Oikonomou, H. Jane Ding, Mohammed Kaplan, Qing Yao, Yi-Wei Chang, Morgan Beeby, Grant Jensen

Nature Communications 10, 1259

De novo structural pattern mining in cellular electron cryotomograms

Min Xu, Jitin Singla, Elitza Tocheva, Yi-Wei Chang, Raymond Stevens, Grant Jensen, Frank Alber

Structure 27 (4), 679-691

In situ imaging and structure determination of bacterial toxin delivery systems using electron cryotomography

Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, Grant J. Jensen

Methods in Molecular Biology 1921, 249-265


In vivo structures of the Helicobacter pylori cag type IV secretion system

Yi-Wei Chang*, Carrie Shaffer*, Lee Rettberg, Debnath Ghosal, Grant Jensen

Cell Reports 23 (3), 673-681


Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

Ariane Briegel, Catherine Oikonomou, Yi-Wei Chang, Andreas Kjær, Audrey Huang, Ki Woo Kim, Debnath Ghosal, Hong Nguyen, Dorothy Kenny, Rachel Ogorzalek Loo, Robert Gunsalus, Grant Jensen

EMBO Reports 18 (9), 1660-1670

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

Yi-Wei Chang, Andreas Kjær, Davi Ortega, Gabriela Kovacikova, John Sutherland, Lee Rettberg, Ronald Taylor, Grant Jensen

Nature Microbiology 2 (4), 16269


Polar delivery of Legionella type IV secretion system substrates is essential for virulence

Kwangcheol Jeong, Debnath Ghosal, Yi-Wei Chang, Grant Jensen, Joseph Vogel

Proceedings of the National Academy of Sciences 114 (30), 8077-8082


Uncharacterized bacterial structures revealed by electron cryotomography

Megan Dobro, Catherine Oikonomou, Aidan Piper, John Cohen, Kylie Guo, Taylor Jensen, Jahan Tadayon, Joseph Donermeyer, Yeram Park, Benjamin Solis, Andreas Kjær, Andrew Jewett, Alasdair McDowall, Songye Chen, Yi-Wei Chang, Jian Shi, Poorna Subramanian, Cristina Iancu, Zhuo Li, Ariane Briegel, Elitza Tocheva, Martin Pilhofer, Grant Jensen

Journal of Bacteriology, JB. 00100-17


In vivo structures of an intact type VI secretion system revealed by electron cryotomography

Yi‐Wei Chang, Lee Rettberg, Davi Ortega, Grant Jensen

EMBO Reports, e201744072


In situ structure of the Legionella Dot/Icm type IV secretion system by electron cryotomography

Debnath Ghosal, Yi‐Wei Chang, Kwangcheol Jeong, Joseph Vogel, Grant Jensen

EMBO Reports 18 (5), 726-732


Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

Qing Yao, Andrew Jewett, Yi‐Wei Chang, Catherine Oikonomou, Morgan Beeby, Cristina Iancu, Ariane Briegel, Debnath Ghosal, Grant Jensen

The EMBO Journal, e201696235


Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium

Emöke Cserti, Sabine Rosskopf, Yi‐Wei Chang, Sabrina Eisheuer, Lars Selter, Jian Shi, Christina Regh, Ulrich Koert, Grant Jensen, Martin Thanbichler

Molecular Microbiology 103 (5), 875-895


Architecture of the type IVa pilus machine

Yi-Wei Chang, Lee Rettberg, Anke Treuner-Lange, Janet Iwasa, Lotte Søgaard-Andersen, Grant Jensen

Science 351 (6278), aad2001


A new view into prokaryotic cell biology from electron cryotomography

Catherine Oikonomou, Yi-Wei Chang, Grant Jensen

Nature Review Microbiology 14, 205-220


Electron cryotomography

Catherine Oikonomou, Matthew Swulius, Ariane Briegel, Morgan Beeby, Qing Yao, Yi-Wei Chang, Grant Jensen

Methods in Microbiology 43, 115-139


Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Yi-Wei Chang, Songye Chen, Elitza Tocheva, Anke Treuner-Lange, Stephanie Löbach, Lotte Søgaard-Andersen, Grant Jensen

Nature Methods 11 (7), 737


Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

Ariane Briegel, Mark Ladinsky, Catherine Oikonomou, Christopher Jones, Michael Harris, Daniel Fowler, Yi-Wei Chang, Lynmarie Thompson, Judith Armitage, Grant Jensen

Elife 3, e02151


Structural basis of a physical blockage mechanism for the interaction of response regulator PmrA with connector protein PmrD from Klebsiella pneumoniae

Shih-Chi Luo, Yuan-Chao Lou, Mahalingam Rajasekaran, Yi-Wei Chang, Chwan-Deng Hsiao, Chinpan Chen

Journal of Biological Chemistry 288 (35), 25551-25561


Crystal Structure of DnaK Protein Complexed with Nucleotide Exchange Factor GrpE in DnaK Chaperone System INSIGHT INTO INTERMOLECULAR COMMUNICATION

Ching-Chung Wu, Vankadari Naveen, Chin-Hsiang Chien, Yi-Wei Chang, Chwan-Deng Hsiao

Journal of Biological Chemistry 287 (25), 21461-21470


Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum

Yi-Wei Chang*, Tai-Wen Lin*, Yi-Chuan Li, Yu-Shan Huang, Yuh-Ju Sun, Chwan-Deng Hsiao

Journal of Biological Chemistry 287 (7), 4783-4789


Structural fold, conservation and Fe (II) binding of the intracellular domain of prokaryote FeoB

Kuo-Wei Hung*, Yi-Wei Chang*, Edward Eng*, Jai-Hui Chen, Yi-Chung Chen, Yuh-Ju Sun, Chwan-Deng Hsiao, Gang Dong, Krasimir Spasov, Vinzenz Unger, Tai-huang Huang

Journal of Structural Biology 170 (3), 501-512


Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1

Yi-Wei Chang*, Yu-Chien Chuang*, Yu-Chi Ho, Ming-Yuan Cheng, Yuh-Ju Sun, Chwan-Deng Hsiao, Chung Wang

Journal of Biological Chemistry 285 (13), 9962-9970


Crystal structure of the N-terminal domain of Geobacillus kaustophilus HTA426 DnaD protein

Cheng-Yang Huang, Yi-Wei Chang, Wei-Ti Chen

Biochemical and Biophysical Research Communications 375 (2), 220-224


Crystal structures of the 70-kDa heat shock proteins in domain disjoining conformation

Yi-Wei Chang, Yuh-Ju Sun, Chung Wang, Chwan-Deng Hsiao

Journal of Biological Chemistry 283 (22), 15502-15511


Structure of the SARS coronavirus nucleocapsid protein RNA-binding dimerization domain suggests a mechanism for helical packaging of viral RNA

Chun-Yuan Chen, Chung-Ke Chang, Yi-Wei Chang, Shih-Che Sue, Hsin-I Bai, Lilianty Riang, Chwan-Deng Hsiao, Tai-Huang Huang

Journal of Molecular Biology 368 (4), 1075-1086